SN/T 1353-2004 English PDF (SNT1353-2004)

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SN/T 1353-2004: Protocol of inspection and quarantine for import fishmeal
SN/T 1353-2004
SN
ENTRY-EXIT INSPECTION AND QUARANTINE INDUSTRY
STANDARDS OF THE PEOPLE’S REPUBLIC OF CHINA
Protocol of inspection and
quarantine for import fishmeal
ISSUED ON. JUNE 1, 2004
IMPLEMENTED ON. DECEMBER 1, 2004
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the People’s Republic of China
Table of Contents
Foreword ... 3 
1 Scope ... 4 
2 Normative references ... 4 
3 On-site inspection and quarantine ... 5 
4 Sampling ... 5 
5 Sample preparation ... 6 
6 Laboratory inspection and quarantine ... 7 
7 Assessment and disposal of inspection and quarantine results ... 8 
Annex A (Normative) Salmonella test method ... 9 
Annex B (Normative) Test method for total bacterial count ... 13 
Annex C (Normative) Mold count test method ... 16 
Annex D (Normative) Test method for pathogenic vibrio ... 18 
Protocol of inspection and
quarantine for import fishmeal
1 Scope
This Standard specifies the methods for on-site inspection and quarantine for
imported fishmeal, methods for extraction and preparation of inspection and
quarantine samples, laboratory inspection and quarantine, and assessment of
inspection and quarantine results.
This Standard applies to the inspection and quarantine for import fishmeal.
2 Normative references
The provisions in the following documents become the provisions of this
Standard through reference in this Standard. For dated references, the
subsequent amendments (excluding corrections) or revisions do not apply to
this Standard. However, parties who reach an agreement based on this
Standard are encouraged to study if the latest editions of these documents are
applicable. For undated references, the latest editions apply to this Standard.
GB/T 5009.45 Method for analysis of hygienic standard of fish and other
aquatic products
GB/T 6432 Determination of crude protein in feeds
GB/T 6433 Determination of crude fat in feeds
GB/T 6435 Method for the determination of moisture in feedstuffs
GB/T 6438 Method for the determination of crude ash in feedstuffs
GB/T 6439 Determination of water-soluble chlorides in feeds
GB/T 9825 Inspection of grain and oils - Determination of insoluble dietary
fiber in cereals
GB/T 18088 Sampling for entry and exit animal quarantine
SN/T 0800.1 Inspection of cereals oils and feedstuffs for import and export -
Methods of sampling and preparation of samples
bag per 50kg, and 50 bags below 100t; in case of above 100t, TAKE 1% of the
total number of bags in batches, SAMPLE 15g per bag, and TAKE one original
sample per 500t (calculated as 500t in case of less than 500t).
4.4.2 Quarantine samples of bagged fishmeal shall be taken as one bag per
50kg, and 40 bags below 100t; in case of above 100t, TAKE 1% of the total
number of bags in batches. Each bag is sampled at 15g, and the collected
samples from 20 bags are integrated into one sample, with a sample weight of
about 300g (calculated as 500t in case of less than 500t).
4.4.3 Inspection and quarantine samples of bulk fishmeal shall be taken
according to 4.4.1 and 4.4.2 after the fishmeal is filled into the warehouse.
4.5 Sampling method
4.5.1 The number of samples is calculated according to 4.4. Samples are
randomly taken around each stack, as well as at the upper, middle and lower
parts in a curved pattern.
4.5.2 The sampling port is faced down, and inserted into the bag from the
diagonal direction of the angle of fishmeal bag, then rotate it upwards to pull it
out, and put it into the sampling bag.
5 Sample preparation
5.1 Tools
USE a cleaned, dry, and sterilized aluminum or a stainless steel sample shovel
(two sheets of thin iron plate or stainless steel plate).
5.2 Methods
5.2.1 Quartering. EXECUTE according to SN/T 0800.1.
5.2.2 The quarantine sample is made into a laboratory sample every 100t; the
test sample is made into a laboratory sample every 3000t.
5.3 Identification
The sample label shall indicate the inspection number, product name, quantity
(weight), inspection items, sample delivery date, sample delivery organization,
etc.
5.4 Sample retention
The samples shall be sealed and stored in a cool and dry place.
Annex A
(Normative)
Salmonella test method
A.1 Pre-enrichment culture
TAKE 25g of quarantine sample and add it to a 500mL jar containing 225mL of
buffered peptone water (the block is granulated with a homogenizer and
crushed for 1min at 8000 r/min~10000 r/min). INCUBATE at 36°C ± 1°C for 16h-
20h.
A.2 Selective enrichment culture
10mL of pre-enrichment culture is taken and inoculated into a culture flask
containing 100mL of TTB enrichment broth. Another 10mL of pre-enrichment
culture is inoculated into a culture flask containing 100mL of SC enrichment
broth. The TTB culture flask is incubated at 43°C for 24h. The SC culture flask
is incubated at 36°C ± 1°C for 24h.
A.3 Quick screening
SELECT commercially available “Salmonella rapid detection kit" or related
microbiological testing instrument supporting reagents or similar commercial
reagents for rapid screening of Salmonella, according to the relevant
commercial instructions, TREAT and TEST TTB and SC selective enrichment
cultures and DETERMINE the positive or negative reaction results. For
negative results, "Salmonella not detected" can be reported; positive results
must be isolated, cultured and identified.
A.4 Isolated culture
A.4.1 TAKE the TTB or SC selective enrichment culture, which is positive in
Chapter A.3, the inoculating loop, and INSCRIBE the phenol red brilliant green
(BS) agar plates and DHL agar plates, respectively, and INOCULATE two plates
each. The bottom of the plate is incubated at 36°C ± 1°C upward. If necessary,
REPEAT the isolated culture of the selective enrichment culture in Chapter A.2.
A.4.2 After isolated culture for 20h-24h, CHECK for the typical colonies of
Salmonella in the plate, the typical colonies of Salmonella grown on BS agar,
TURN the color of the medium change from pink to red, and the colonies are
red and transparent. A typical colony of Salmonella grown on DHL medium is a
yellow-brown transparent, black in center, or a small yellow-brown colony.
becomes purple after growth, indicating a positive reaction. About 95% of the
Salmonella reactions are positive.
A.6 Antigen reaction
A.6.1 The presence of the antigen is examined by pure culture of colonies
using Salmonella factor serum O, Vi or H type by plate agglutination. On a
carefully cleaned glass plate, a drop of saline is placed to partially disperse the
colonies in the saline. After mixing evenly, gently SHAKE for 30s-60s and
OBSERVE the black shadow. If the bacteria have condensed into more or less
clear units, this strain is considered to be self-coagulated and shall not be
provided for antigen identification.
A.6.2 O antigen inspection. Using pure colonies considered to have no self-
coagulation, REPLACE the saline with 1 drop of O-type serum according to the
method of A.6.1. If agglutination occurs, it is judged as positive.
A.6.3 Vi antigen inspection. Using pure colonies considered to have no self-
coagulation, REPLACE the saline with 1 drop of Vi-type serum according to the
method of A.6.1. If agglutination occurs, it is judged as positive.
A.6.4 H antigen inspection. Pure colonies which are considered to have no
self-coagulation are inoculated in semi-solid nutrient agar and cultured at 36°C
± 1°C for 1...