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GB 29989-2013: Determination of L-carnitine in infant foods and dairy products
GB 29989-2013
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard -
Determination of Levocarnitine in Foods and
Milk Products for Infants and Young Children
ISSUED ON. NOVEMBER 29, 2013
IMPLEMENTED ON. JUNE 1, 2014
Issued by. National Health and Family Planning Commission of the
People's Republic of China
Table of Contents
1 Scope ... 3 
2 Principle ... 3 
3 Reagents and Materials ... 3 
4 Apparatuses and devices ... 5 
5 Analytical Procedures ... 5 
6 Expression of analytical result ... 6 
7 Precision ... 6 
8 Other ... 6 
National Food Safety Standard -
Determination of Levocarnitine in Foods and Milk
Products for Infants and Young Children
1 Scope
This Standard specifies the method for determination of levocarnitine in foods and
milk products for infants and young children.
This Standard is applicable to the determination of levocarnitine in foods and milk
products for infants and young children.
2 Principle
Extract the specimen with water; use perchloric acid to precipitate the protein; then
filtrate it. After the filtrate is gone through alkaline saponification, the combined-state
levocarnitine in solution is dissociated. Under the catalysis of carnitine acetyl
transferase, levocarnitine reacts with acetyl coenzyme A to generate acetylcarnitine
and free coenzyme A. Free coenzyme A reacts with 2-nitrobenzoic acid to generate
yellow substance; the color of which is proportional to the content of free coenzyme A.
As the free coenzyme A and levocarnitine are of same mole reaction, the content of
levocarnitine in specimen may be calculated indirectly.
3 Reagents and Materials
Note. Unless otherwise specified, all reagents adopted in this method are analytical reagents
and the water is Grade 3 specified in GB/T 6682.
3.1 Reagents
3.1.1 Perchloric acid (HClO4).
3.1.2 Sodium hydroxide (NaOH).
3.1.3 Potassium hydroxide (KOH).
3.1.4 2-nitrobenzoic acid (C14H8N2O8S2).
3.1.5 N-2-hydroxyethyl piperazine-N-2-ethanesulfonic acid (C8H18N2O4S).
6 Expression of analytical result
The content X of levocarnitine in specimen is expressed by mass fraction mg/100g
and calculated according to Formula (1).
cX
m V
   ... (1)
Where,
X - the content of levocarnitine in specimen, mg/100g;
c - the concentration of specimen treatment solution obtained through standard
curve, μg/mL;
V - the filtrate volume, mL;
m - the specimen mass, g.
The result is expressed as the arithmetic mean of two independent determination
results under repeatability conditions, accurate to one decimal place.
7 Precision
The absolute deviation of two independent determination results under repeatability
conditions shall not exceed 10% of arithmetic mean.
8 Other
Method detection limit is 0.6mg/100g; quantitation limit is 2mg/100g.