GB/T 19495.7-2004 English PDF (GBT19495.7-2004)

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GB/T 19495.7-2004: Detection of genetically modified organisms and derived products -- Methods for sampling and sample preparation
GB/T 19495.7-2004
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 67.050
B 04
Detection of genetically modified organisms
and derived products - Methods for
sampling and sample preparation
ISSUED ON. APRIL 13, 2004
IMPLEMENTED ON. APRIL 13, 2004
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the PRC;
Standardization Administration of the PRC.
Table of Contents
Foreword ... 3
1 Scope ... 5
2 Normative references ... 5
3 Terms and definitions ... 5
4 General ... 7
5 Sampling, sample preparation tools and equipment ... 8
6 Sampling size ... 8
7 Sampling methods ... 11
8 Methods for sample preparation ... 14
9 Packaging, identification, and transport of sample ... 16
10 Sampling report ... 16
11 Restore sample ... 17
12 Health and safety ... 18
Foreword
GB/T 19495 “Detection of genetically modified organisms and derived products”
is a series of standards.
- GB/T 19495.1-2004 Detection of genetically modified organisms and
derived products - General requirements and definitions;
- GB/T 19495.2-2004 Detection of genetically modified organisms and
derived products - General requirements for laboratories;
- GB/T 19495.3-2004 Detection of genetically modified organisms and
derived products - Nucleic acid extraction;
- GB/T 19495.4-2004 Detection of genetically modified organisms and
derived products - Qualitative nucleic acid based methods;
- GB/T 19495.5-2004 Detection of genetically modified organisms and
derived products - Quantitative nucleic acid based methods;
- GB/T 19495.6-2004 Detection of genetically modified organisms and
derived products - Gene-chip detection;
- GB/T 19495.7-2004 Detection of genetically modified organisms and
derived products - Methods for sampling and sample preparation;
- GB/T 19495.8-2004 Detection of genetically modified organisms and
derived products - Protein based methods.
This Part was proposed by and shall be under the jurisdiction of Certification
and Accreditation Administration of the PRC.
This Part is approved and issued by General Administration of Quality
Supervision, Inspection and Quarantine of the PRC.
Main drafting organization of this Part. Liaoning Entry-Exit Inspection and
Quarantine Bureau of the PRC.
Participating drafting organizations of this Part. Laboratory of Animal and Plant
Quarantine of General Administration of Quality Supervision, Inspection and
Quarantine, Heilongjiang Entry-Exit Inspection and Quarantine Bureau of the
PRC, Guangdong Entry-Exit Inspection and Quarantine Bureau of the PRC,
Shenzhen Entry-Exit Inspection and Quarantine Bureau of the PRC, Shanghai
Entry-Exit Inspection and Quarantine Bureau of the PRC, South China
Agricultural University, Institute of Plant Protection of Shandong Academy of
Agricultural Sciences.
Detection of genetically modified organisms
and derived products - Methods for
sampling and sample preparation
1 Scope
This Part of GB/T 19495 specifies methods for sampling and sample
preparation for the detection of genetically modified products in plants and their
products.
This Part applies to the sampling and sample preparation of plants and their
products for the detection of genetically modified products.
2 Normative references
The clauses in the following documents, by reference in this Part of GB/T 19495,
constitute the clauses of this Part. For the dated references, the subsequent
amendments (not including errata content) or revisions do not apply to this Part.
However, parties which have reached an agreement according to this Part are
encouraged to study whether the latest edition of these documents can be used.
For the undated references, the latest edition is applicable to this Part.
GB/T 19495.1-2004 Detection of genetically modified organisms and derived
products - General requirements and definitions
GB/T 19495.2-2004 Detection of genetically modified organisms and derived
products - General requirements for laboratories
GB/T 19495.3-2004 Detection of genetically modified organisms and derived
products - Nucleic acid extraction
3 Terms and definitions
The following terms and definitions apply to this Part of GB/T 19495.
3.1 Consignment
Plants or plant products submitted, shipped, or received at one time, which may
consist of one or more lots.
3.11 Test sample, analytical sample
The sample for analysis or testing obtained by further homogenization of all or
part of the laboratory sample.
3.12 Restore sample
A certain number of samples taken from laboratory samples for future reference.
3.13 Acceptable GMO quality level
The acceptable amount of genetically modified products contained in the
specified consignment at the time of sampling acceptance, which is generally
expressed in mass percentage content.
4 General
4.1 General provisions
4.1.1 Samples taken and prepared shall be representative.
4.1.2 It shall ensure that sampling instruments are clean, dry, and have no odor.
The materials used in the sample preparation instruments and sample
containers shall not contaminate the sample to be taken.
4.1.3 To avoid cross contamination, it shall as far as possible use different
sampling and sample preparation instruments or equipment to extract and
prepare samples of different consignments; containers or packaging containing
samples shall be used as single as possible. If this is not possible (for example,
when using mechanical sampling equipment), after extracting and preparing
the sample of a consignment, all instruments and equipment shall be cleaned
using appropriate methods.
During all sampling processes, samples shall not be scattered, to prevent active
organisms from polluting the ecological environment.
Samples shall be prepared in physically isolated areas, to prevent
contamination of other areas or laboratories. The sample preparation area shall
be cleaned in time.
When necessary, USE DNA destruction agent to process sampling and sample
preparation instruments and equipment, sample containers, and sample
preparation areas.
When applicable, it shall refer to the provisions in GB/T 19495.1-2004 to
prevent pollution.
4.1.4 When sampling, samples shall be protected. Sampling instruments and
sample containers shall be stored in a clean environment, to avoid
contamination from foreign objects such as rain and dust.
4.1.5 All sampling operations shall be completed in the shortest possible time,
to avoid changes in the composition of sample. If a sampling step takes a long
time, the sample shall be stored in a closed container.
4.2 Lot grouping
First, according to the same contract, the same production lot number, the same
variety, and grade in the consignment, it shall be lotted. When the consignment
size is less than 10000 t, the maximum lot size is 500 t. When the consignment
size is greater than 10000 t, 10000 t divided into 20 lots is the base. For every
extra 1000 t, add 1 lot; if less than 1000 t, counted as 1 lot.
The consignment size divided by the number of lots is the lot size. For example,
a consignment of 600 t is divided into 2 lots, the lot size is 300 t. A consignment
of 13200 t is divided into 24 lots, the lot size is 550 t.
5 Sampling, sample preparation tools and equipment
According to the characteristics of the sample to be taken, sampling, sample
preparation instruments and equipment complying with the requirements of ...