GB 20287-2006 English PDF (GB20287-2006)

Delivery: 3 seconds. Download true-PDF + Invoice.
Get QUOTATION in 1-minute: Click GB 20287-2006
Historical versions: GB 20287-2006
Preview True-PDF (Reload/Scroll if blank)

GB 20287-2006: Microbial inoculants in agriculture
GB 20287-2006
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.100.01
B 13
Microbial inoculants in agriculture
ISSUED ON: MAY 25, 2006
IMPLEMENTED ON: SEPTEMBER 01, 2006
Issued by: General Administration of Quality Supervision, Inspection and
Quarantine of PRC;
Standardization Administration of PRC.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Terms and definitions ... 4
4 Product classification ... 5
5 Requirements ... 5
5.1 Bacteria species ... 5
5.2 Product appearance (sensory) ... 5
5.3 Product technical index ... 5
6 Test method ... 7
6.1 Instrument and equipment... 7
6.2 Reagents ... 7
6.3 Testing of product parameters ... 7
7 Inspection rules ... 12
7.1 Sampling ... 12
7.2 Inspection classification ... 13
7.3 Judgment rules ... 14
8 Packaging, marking, transportation, storage ... 14
8.1 Packaging ... 14
8.2 Markings ... 15
8.3 Transportation ... 16
8.4 Storage ... 16
Appendix A (Normative) Commonly used testing medium ... 17
Appendix B (Informative) Commonly used stains ... 22
Appendix C (Normative) Dilution method (MPN 5-tube method)... 24
Appendix D (Normative) Determination of enzyme activity ... 26
Microbial inoculants in agriculture
1 Scope
This standard specifies the terms and definitions, product classification,
requirements, test methods, inspection rules, packaging, marking,
transportation, storage of microbial inoculants in agriculture (i.e., microbial
inoculants).
This standard applies to microbial inoculum products in agriculture.
2 Normative references
The provisions in following documents become the provisions of this Standard
through reference in this Standard. For the dated references, the subsequent
amendments (excluding corrections) or revisions do not apply to this Standard;
however, parties who reach an agreement based on this Standard are
encouraged to study if the latest versions of these documents are applicable.
For undated references, the latest edition of the referenced document applies.
GB/T 1250-1989 Rules for expression and judgement of limiting values
GB/T 8170 Rules of rounding off for numerical values and expression and
judgement of limiting values
GB 18877-2002 Organic-inorganic compound fertilizers
GB/T 19524.1 Determination of fecal coliforms in fertilizers
GB/T 19524.2 Determination of mortality of ascarid egg in fertilizers
QB/T 1803-1993 General methods of determination for industrial enzymes
3 Terms and definitions
The following terms and definitions apply to this standard.
3.1
Microbial inoculants in agriculture
The live bacteria preparations, which are processed from target
If the dilution method [Most Probable Number (MPN) 5 pipe method] is used, it
is performed in accordance with the provisions of Appendix C.
6.3.2.1 Serial dilution
Weigh 10 g of the sample (accurate to 0.01 g). Add 100 mL of sterile water (or
physiological saline), which contains glass beads [for liquid inoculants, take
10.0 mL AND add 90 mL of sterile water (or physiological saline)]. Let it stand
for 20 min. Fully shake it on a rotary shaker at 200 r/min for 30 min, to form a
mother liquor bacterial suspension (basic liquid).
Use a sterile pipette to pipette 5.0 mL of the above-mentioned mother liquor
bacterial suspension. Add 45 mL of sterile water (or physiological saline).
Perform serial dilutions according to 1→10, to respectively obtain the bacterial
suspension of the dilution 1→1 x 101, 1→1 x 102, 1→1 x 103, 1→1 x 104... …
(the sterile pipette shall be replaced for each dilution).
6.3.2.2 Sample addition and cultivation
Take 3 consecutive suitable dilutions for each sample. Use a sterile pipette to
respectively pipette 0.1 mL of the bacterial suspension of different dilutions. Add
them to the pre-prepared solid medium plate. Respectively use a sterile glass
spatula, to uniformly coat the bacterial suspension, of different dilutions, on the
surface of the agar.
Each dilution is repeated 3 times. At the same time, use the sterile water (or
physiological saline) as a blank control. Culture it under appropriate conditions.
6.3.2.3 Colony identification
According to the technical data of the tested bacteria, for each dilution, take
different types of representative colonies, to confirm the effective bacteria,
through technical means such as smears, staining, microscopy. When the count
of colonies appears in the blank control cultural dish, the test result is invalid.
The test shall be repeated.
6.3.2.4 Colony count
Take the dilution plate, which appears 20 ~ 300 colonies, as the counting criteria
(10 ~ 150 colonies for filamentous fungi). Respectively count the number of
effective viable bacteria and the number of miscellaneous bacteria. When there
is only one dilution AND the average number of colonies is between 20 ~ 300,
the average number of colonies is used for calculation. If there are two dilutions,
when the average count of colonies is between 20 ~ 300, it shall be determined,
according to the ratio of the total number of the two colonies. If the ratio is less
than or equal to 2, it shall calculate the average number of the two. If it is greater
than 2, the average number of colonies, which have smaller dilution, shall be
instrument reading is stable.
6.3.7.3 Particle samples
The sample is first crushed and pass through a 1.0 mm test sieve. Make
measurement, according to the method of 6.3.7.2.
6.3.8 Determination of the number of fecal coliforms
It shall meet the requirements of GB/T 19524.1.
6.3.9 Determination of the mortality of ascaris eggs
It shall meet the requirements of GB/T 19524.2.
6.3.10 Determination of cellulase activity and protease activity
It shall meet the requirements of Appendix D.
6.3.11 Determination of arsenic, cadmium, lead, chromium, mercury
It shall meet the requirements of 5.12 ~ 5.17 in GB 18877-2002.
6.3.12 Inspection of shelf life
Before the shelf life indicated in the product manual, measure the
corresponding indicators of the product, according to the methods 6.3.1 ~
6.3.11.
7 Inspection rules
The digital rounding of product technical index in this standard shall comply with
the provisions of GB 8170. The qualification judgment of product quality shall
comply with the provisions of the comparison method of rounding values in GB
1250-1989.
7.1 Sampling
The products, which are processed from each fermenter's bacteria liquid (or
each batch of solid fermentation), as a batch, to carry out sampling inspection.
During the sampling process, it shall strictly avoid the contamination of bacteria.
7.1.1 Sampling tools
Sterile plastic bags (bottles), metal spoons, samplers, measuring cylinders,
kraft paper bags, glue, sampling seals, sampling sheets, etc.
7.3 Judgment rules
7.3.1 Any products, which meet the requirements of one of the following
clauses, are qualified products
a) Products whose technical index meet the requirements of the standard;
b) In the product appearance, moisture, fineness, pH value and other test
items, if there is 1 item that does not meet the requirements, BUT the other
technical index meet the requirements.
7.3.2 Any products, which meet the requirements of one of the following
clauses, are unqualified products
a) The number of effective viable bacteria does not meet the technical
indicators;
b) The number of molds and miscellaneous bacteri...