QB/T 2881-2013 English PDF (QBT2881-2013)

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QB/T 2881-2013: Footwear and footwear components - Antimicrobial performance specifications
QB/T 2881-2003
QB
INDUSTRY STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 61.060
Y 28
File number. 43565-2013
Replacing QB/T 2881-2007
Footwear and footwear components -
Antibacterial performance specifications
ISSUED ON. DECEMBER 31, 2013
IMPLEMENTED ON. JULY 01, 2014
Issued by. Ministry of Industry and Information Technology of the
People's Republic of China
Table of Contents
Foreword ... 3 
1  Scope ... 5 
2  Normative references ... 5 
3  Terms and definitions ... 5 
4  Test environment ... 6 
5  Sampling ... 6 
6  Test strains ... 6 
7  Test methods ... 6 
8  Requirements ... 7 
9  Judgment ... 8 
10  Identification ... 8 
11  Report ... 8 
Annex A ... 9 
Annex B ... 12 
Annex C ... 17 
Annex D ... 20 
Annex E ... 23 
Foreword 
This standard is drafted in accordance with the provisions given in GB/ T 1.1-2009.
This standard is the revision of QB/T 2881-2007 “Antibacterial Technical Requirements for
Footwear Linings and Insoles”. Compared with QB/T 2881-2007, the main technical
changes are as follows.
— Change the standard name from “Antibacterial Technical Requirements For Footwear
Linings and Insoles” to “Footwear and Footwear Components - Antibacterial
Performance Specifications”;
— Expand the standard scope;
— Add and change the normative application documents;
— Add the terms and definitions of antibacterial shoes;
— Add the requirements for product identification;
— Add the sampling requirements;
— Modify the antibacterial test strains.
— Add the dissolution method and oscillation method. They are respectively as annex A
and annex D;
— Add the dissolution indicators in requirements;
— Add the requirements for antibacterial agent in selection, which shall not affect the
environment after product disposal;
— Adjust the indicators in table 1 of previous standard;
— Add Chapter 9 “Judgment”.
This standard was proposed by China National Light Industry Council.
This standard shall be under the jurisdiction of National Technical Committee on Footwear
of Standardization Administration of China (SAC/TC 305).
Drafting organizations of this standard. ANTA (CHINA) CO., LTD., China Leather and
Footwear Industry Research Institute, Fujian Nan'an Bangdeng Shoes Industry Co., Ltd.,
Fujian Zhangping Yingchuan Light Industrial Co., Ltd., and Jinda Nano Tech (Xiamen) Co.,
Ltd.
Main drafters of this standard. Li Su, Zhang Weijuan, Hou Jingguo, Chen Junyuan, Wu
Footwear and footwear components –
Antibacterial performance Specifications
Warning. The test methods specified in this document require the use of related
microorganisms. Only professionals who receive the training on microbiology and
have practical experience can use microbiological testing method of this standard
in laboratory with microbial treatment specifications. In consideration of laws and
regulations of specific countries, appropriate safety measures shall be taken.
1  Scope 
This standard specifies the terms and definitions, test environment, sampling, test strains,
test method, requirements, determination, identification, and report of antibacterial
performances of footwear and footwear components.
This standard applies to footwear and footwear components with antibacterial
performance.
2  Normative references 
The articles contained in the following documents have become part od this document
when they are quoted herein. For the dated documents so quoted, all the modifications
(Including all corrections) or revisions made thereafter shall be applicable to this
document.
GB 4789.2 National food safety standard Food microbiological examination. Aerobic
plate count
GB/T 8629-2001 Textiles - Domestic washing and drying procedures for textile testing
GB 19489 Laboratories - General requirements for biosafety
3  Terms and definitions 
For the purpose of this standard, the following terms and definitions apply.
3.1
Antibacterial
Annex A 
(Normative)
Dissolution Test Method (Inhibition-Zone Test)
A.1 Principle
Use antibacterial agent to dissolve out constantly, and form different concentration
gradients through agar diffusion, so as to show bacteriostatic action. The test is judged by
whether antibacterial agent dissolves out or conforms to dissolution requirements of this
standard based on inhibition-zone size.
A 2 Test conditions
A.2.1 Main equipment
Constant temperature incubator (37±1)°C; freezer 5°C~10°C; second-level biological
safety cabinet; pressure steam sterilizer; electrically heated drying oven; Vernier calliper;
sterile test tubes; sterile pipette; sterile Erlenmeyer flask; inoculating loop; and alcohol
lamp.
A.2.2 Preparation of materials
A.2.2.1 Control samples
It shall be the sterile dry filter paper in round of which the diameter is 15 mm, or in square
of which the side length is 15 mm.
A.2.2.2 Samples
It shall be the suitable test samples in round of which the diameter is 15 mm, or in square
of which the side length; the thickness is less than 4mm; cut from the antibacterial parts.
A 2.2.3 Sterilization
Sterilize the test sample and control sample before testing. Samples that are not suitable
for treatment with disinfectant may be dried directly after being rinsed with sterile water
and receiving ultraviolet irradiation for 1 h. Other instruments used in test may be
sterilized through hot and humid method OR hot and dry method.
A.2.3 Culture medium and reagents
A.2.3.1 Nutrient broth (NB)
Add 5.0 g of beef extract, 10.0 g of peptone, and 5.0 g of sodium chloride to 1000 mL of
distilled water. After dissolution by heating, adjust the pH through 0.1 mol/L sodium
Take 0.3 mL of bacterial solution that is prepared based on A.3.3; apply evenly to culture
medium; cover with plate; stand for 5 min.
A.4.2 Sample placing
Paste to 3 contamination plates; each plate is pasted with 2 test samples and 2 control
samples. Use sterile forceps to paste test sample to plate surface; sample center distance
is less than 25 mm, and the distance from plate edge is not less than 15 mm. After pasting
in place, use sterile forceps to gently press the samples so that the samples are very
close to surface of the plate.
A.4.3 Culture and measurement
Cover the dish and cultivate at (37±1)°C for 16 h~18 h. Use Vernier calliper to measure
inhibition-zone (including sample) and record. Note that growth of some microbes may
restore and make bacteriostasis circle smaller if incubation time is too long.
Full transparent inhibition-zone (the widest place in normal direction) shall be selected for
inhibition-zone measurement. Measurement diameter shall be bounded by outer edge of
inhibition-zone.
A.5 Expression of test results
A.5.1 Control sample shall not generate inhibition-zone, otherwise the test is invalid.
A.5.2 Calculate the inhibition-zone width (D) based on formula (A.1); take arithmetic mean
value of 3 samples, and retain 2 significant figures.
Where,
D — Bacteriostatic ring width, in millimeters (mm)；
T — Total width of bacteriostatic ring outer edge, in millimeter (mm);
R — Total width of test sample, in millimeter (mm).
B.2.2.3 Sterilization
High-pressure steam sterilization is usually adopted at 115°C for 30 min. For the test
samples that are not heat-resisting or that hot and humid disinfection method may easily
affect antibacterial performance, other suitable methods may also be used for sterilization...