GB 1886.317-2021 English PDF (GB1886.317-2021)

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GB 1886.317-2021: National food safety standard - Food additives - β-Carotene (sourced from Dunaliella)
GB 1886.317-2021
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard –
Food Additives - β-Carotene (Sourced from Dunaliella)
ISSUED ON: FEBRUARY 22, 2021
IMPLEMENTED ON: AUGUST 22, 2021
Issued by: National Health Commission of the People’s Republic of China;
State Administration for Market Regulation.
Table of Contents
1 Scope ... 3
2 Chemical Name, Molecular Formula, Structural Formula, and Relative
Molecular Mass ... 3
3 Technical Requirements ... 3
Appendix A Inspection Methods ... 5
National Food Safety Standard –
Food Additives - β-Carotene (Sourced from Dunaliella)
1 Scope
This Standard is applicable to take the Dunaliella Salina as the raw materials, and
obtain the food additives of β-Carotene (sourced from Dunaliella) through extracting
and refining.
2 Chemical Name, Molecular Formula, Structural
Formula, and Relative Molecular Mass
2.1 Chemical name
1,1'-(3,7,12,16-tetramethyl-1,3,5,7,9,11,13,15,17-octadecenone-1,18-diyl) double
[2,6,6-trimethylcyclohexene]
2.2 Molecular formula
C40H56
2.3 Structural formula
2.4 Relative molecular mass
536.89 (as per 2018 international relative atomic mass)
3 Technical Requirements
3.1 Sensory requirements
The sensory requirements shall comply with the provisions of Table 1.
Appendix A
Inspection Methods
A.1 General provisions
If no other requirements are specified, all the reagents and water used in this Standard
refer to the analytical reagents and Class-III water specified in GB/T 6682. If no other
requirements are specified, all the standard solution used in the test, standard solution
for determining the impurity, preparation and product shall be prepared according to
the provisions of GB/T 601, GB/T 602, GB/T 603.The solution used in the test refers
to the aqueous solution when the solvent is not specified.
A.2 Identification test
A.2.1 Maximum absorption wavelength
Measured by a spectrophotometer, the specimen's cyclohexane solution (5mg/L) has
maximum absorption values at 448nm~457nm and 474nm~486nm.
A.2.2 Colour reaction
Drop the specimen toluene solution (about 400μg/mL) on the filter paper; spray by 20%
antimony trichloride toluene solution; and it turns blue after 2min ~ 3min.
A.3 Determination of the total natural carotenoids content
A.3.1 Principle of the method
β-carotene is a compound with conjugated double bonds. It has a maximum absorption
value at a wavelength of 455nm±1nm, which can be used to determine the total
carotenoid content. The specimen is dissolved in chloroform and diluted with hexane
to an appropriate concentration. The final dilution is carried out in cyclohexane. The
absorbance is measured and the concentration is calculated by the absorbance
coefficient.
A.3.2 Reagents and materials
A.3.2.1 Chloroform.
A.3.2.2 Cyclohexane.
A.3.2.3 Hexane.
A.3.3 Apparatus
A.4.4 Analysis procedures
A.4.4.1 Preparation of test solution
A.4.4.1.1 γ-terpinene internal standard stock solution
Accurately weigh 1.0g of γ-terpinene standard product, accurate to 0.0001g; dissolve
in n-hexane; transfer it into a 100mL volumetric flask; use n-hexane to make constant
volume to the mark; shake well for later-use.
A.4.4.1.2 d-limonene standard stock solution
Accurately weigh 0.5g (accurate to 0.0001g) of d-limonene standard product; dissolve
in n-hexane; transfer to a 50mL volumetric flask; use n-hexane to make constant
volume to the mark; shake well for later-use.
A.4.4.1.3 d-limonene standard working solution
Accurately pipette 5.00mL of d-limonene standard stock solution into a 50mL
volumetric flask; use n-hexane to make constant volume to the mark; shake well for
later-use.
A.4.4.1.4 Serial standard working solution
Accurately pipette 1.00mL of γ -terpinene internal standard stock solution and
10.00mL of d-limonene standard stock solution into a 25mL volumetric flask; dilute to
the mark with n-hexane; shake well for later-use. Accurately pipette 2.00mL of γ-
terpinene internal standard stock solution and 5.00mL of d-limonene standard stock
solution in a 50mL volumetric flask; dilute to the mark with n-hexane; shake well for
later-use. Accurately pipette 2.00mL of γ-terpinene internal standard stock solution
and 5.00mL of d-limonene standard working solution in a 50mL volumetric flask; dilute
to the mark with n-hexane; shake well for later-use. Accurately pipette 2.00mL γ-
terpinene internal standard stock solution and 2.00mL of d-limonene standard working
solution into a 50mL volumetric flask; dilute to the mark with n-hexane; shake well for
later-use. Blank solution, accurately pipette 2.00mL of γ-terpinene internal standard
stock solution into a 50mL volumetric flask; dilute to the mark with n-hexane; shake
well for later-use. Prepared into a serial standard working solutions containing 0 mg/mL,
0.04 mg/mL, 0.1 mg/mL, 1.0 mg/mL, 4.0 mg/mL of d-limonene and 0.4 mg/mL γ-
terpinene, respectively.
A.4.4.2 System stability test
A.4.4.2.1 When the gas chromatograph operates the blank solution for twice, there
shall be no d-limonene chromatographic peak.
A.4.4.2.2 When the gas chromatograph operates the serial standard working solution