YC/T 159-2019 English PDF (YCT159-2019)

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YC/T 159-2019: Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method
YC/T 159-2019
TOBACCO INDUSTRY STANDARD OF
THE PEOPLE’S REPUBLIC OF CHINA
ICS 65.160
X 85
Replacing YC/T 159-2002
Tobacco and tobacco products - Determination of
water soluble sugars - Continuous flow method
ISSUED ON: DECEMBER 26, 2019
IMPLEMENTED ON: JANUARY 15, 2020
Issued by: State Tobacco Monopoly Administration
Table of Contents
Foreword ... 3 
1 Scope ... 5 
2 Normative references ... 5 
3 Principle ... 5 
4 Reagents ... 5 
5 Instruments and materials ... 7 
7 Result calculation and expression ... 8 
8 Precision and recovery rate ... 9 
9 Test report ... 10 
Annex A (informative) Flow chart of continuous flow analysis of water-soluble
sugar... 12 
Foreword
This Standard was drafted in accordance with the rules given in GB/T 1.1-2009
and GB/T 20001.4-2015.
This Standard replaces YC/T 159-2002 “Tobacco and tobacco products -
Determination of water soluble sugars - Continuous flow method”. Compared
with YC/T 159-2002, in addition to editorial modifications, the main technical
changes are as follows:
- modified green/green pipe sodium hydroxide solution concentration (see
4.2 of this Edition, 4.2 of Edition 2002);
- modified reagent for measuring red/red pipe diameter when reducing sugar
(see 4.6 of this Edition, 4.6 of Edition 2002);
- modified 85°C heating tank volume (see 5.1 of this Edition, 5/1 of Edition
2002);
- added heat-dissipation device (see 5.1 of this Edition, 5.1 of Edition 2002).
Please note that some content of this document may involve patents. The issuer
of this document does not assume responsibility for identifying these patents.
This Standard was proposed by State Tobacco Monopoly Administration.
This Standard shall be under the jurisdiction of Subcommittee on Cigarette of
National Technical Committee on Tobacco of Standardization Administration of
China (SAC/TC 144/SC 1).
The drafting organizations of this Standard: National Tobacco Quality
Supervision and Inspection Center, Shanghai Tobacco Group Beijing Cigarette
Factory, Yunnan China Tobacco Industry Co., Ltd., Yunnan China Tobacco
Recycled Tobacco Co., Ltd., Zhengzhou Tobacco Research Institute, Yunnan
Tobacco Quality Supervision and Inspection Station, Guizhou China Tobacco
Industry Co., Ltd., Henan China Tobacco Industry Co., Ltd., Henan Tobacco
Quality Supervision and Inspection Station, Northwest Tobacco Quality
Supervision and Inspection Station, Guangdong Tobacco Quality Supervision
and Inspection Station, Shandong China Tobacco Industry Co., Ltd.
Main drafters of this Standard: He Shengbao, Zhang Wei, Luo Anna, Liu Nan,
Feng Xiaomin, Wang Yingyuan, Ma Yanjun, Qin Yunhua, Liu Enfen, Wang Ran,
Guo Junwei, Peng Qianrong, Li Wenwei, Peng Lijuan, Ma Li, Du Guorong,
Wang Fei, Wang Kai, Peng Yuntie, Shi Hongyan, Liu Wei, Wang Hongbo,
Zhang Jianfeng, Mao Jian, Ji Houwei, Wu Shouming, Guo Shuwen, Zhao
Haijuan.
Tobacco and tobacco products - Determination of
water soluble sugars - Continuous flow method
1 Scope
This Standard specifies the method to determine water-soluble sugar (including
water-soluble total sugar and water-soluble reducing sugar) in tobacco and
tobacco products.
This Standard is applicable to the determination of water-soluble sugar in
tobacco and tobacco products. The detection limit of this method is 0.0063%.
quantitation limit is 0.0210%.
2 Normative references
The following referenced documents are indispensable for the application of
this document. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any
amendments) applies.
GB/T 6682, Water for analytical laboratory use - Specification and test
methods
YC/T 31, Tobacco and tobacco products - Preparation of test sample and
determination of water content - Oven method
3 Principle
Use 5% aqueous acetic acid to extract the sample. The sugar in the extract
reacts with p-hydroxybenzoic acid hydrazide. Produce yellow azo compounds
in alkaline media at 85°C. Its maximum absorption wavelength is 410nm. Use
colorimeter to determine.
4 Reagents
Except for special requirements, analytically pure reagents shall be used. The
water shall comply with the regulations for grade one water in GB/T 6682.
4.1 Polyoxyethylene lauryl ether solution (Brij35 solution)
- 5% p-hydroxybenzoic acid hydrazide solution is yellow;
- It is difficult to dissolve p-hydroxybenzoic acid hydrazide in 0.5mol/L sodium
hydroxide solution;
- There are suspended particles in the solution;
- The baseline is wavy.
4.10 5% p-hydroxybenzoic acid hydrazide solution
Add 250mL of 0.5mol/L hydrochloric acid solution (4.7) to a beaker. Add 25g of
paraben hydrazide (4.9) to make it dissolved. Add 10.5g of citric acid (C6H8O7
• H2O). After dissolution, transfer to a 500mL volumetric flask. Use 0.5mol/L
hydrochloric acid solution to dilute to the scale. Store at 0°C~4°C.
5% p-hydroxybenzoic acid hydrazide solution can also be prepared by the
following method. Add 250mL of 0.5mol/L hydrochloric acid solution (4.7) to the
beaker. Heat to 45°C. Add p-hydroxybenzoic acid hydrazide and citric acid
under continuous stirring. Transfer to volumetric flask after cooling. Use 0.5
mol/L hydrochloric acid solution to dilute to the scale. The p-hydroxybenzoic
acid hydrazide solution prepared in this way can avoid the formation of
precipitates in the pipeline.
4.11 D-glucose, purity ≥99.5%.
4.12 Standard stock solution: Weigh 20.0g of dry D-glucose (4.11) in a beaker,
to the nearest of 0.0001g. Use water to dissolve then transfer into a 500mL
volumetric flask and set volume to the scale. The standard stock solution is
stored in the refrigerator at 0°C~4°C. The validity is 1 month.
4.13 Series standard working solution: Use 5% acetic acid solution (4.4) to
prepare at least 5 standard working solution from stock solution. The
concentration range shall cover the expected sample content. The standard
stock solution is stored in the refrigerator at 0°C~4°C. The validity is 2 weeks.
5 Instruments and materials
5.1 Continuous flow analyzer, consisting of the following parts:
- Sampler;
- Proportional pump;
- Dialyzer;
- Heating tank;
- Spiral tube;
- Colorimeter with 410nm filter;
- Data processing device;
- Heat-dissipation device (radiating fin or equivalent cooling device).
5.2 Balance, with a sensitivity of 0.0001g.
5.3 Oscillator.
5.4 Quantitative filling injector or pipette.
5.5 50mL Erlenmeyer flask with stopper.
5.6 Rapid qualitative filter paper.
6 Analysis steps
6.1 Sample preparation
Prepare sample according to YC/T 31 and measure its moisture content.
6.2 Sample processing
Weigh 0.25g of sample in the 50mL Erlenmeyer flask with stopper (5.5), to the
nearest of 0.0001g. Add 25mL of 5% acetic acid solution (4.4). Cover with a
stopper. Shake on the shaker (5.3) (rotation speed >150r/min) and extract for
30min. Use rapid qualitative filter paper (5.6) to filter the extract. Discard the
first 2mL~3mL of filtrate. Collect subsequent filtrate for analysis.
6.3 Instrument analysis
Operate the series of standard working solution (4.13) and subsequent filtrate
of sample processing (6.2) on the instrument. See Annex A for the analysis
flowchart. If the sample concentration exceeds the standard working solution
concentration range, it shall be diluted before measurement.
7 Result calculation and expression ...