GB 31604.40-2016 English PDF (GB31604.40-2016)

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GB 31604.40-2016: Food contact materials -- Polymer -- Determination of maleic acid and maleic anhydride in food simulants -- High performance liquid chromatography
GB 31604.40-2016
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard -
Food Contact Materials and Articles - Determination of
Migration of Maleic Acid and Its Acid Anhydride
ISSUED ON. OCTOBER 19, 2016
IMPLEMENTED ON. APRIL 19, 2017
Issued by. National Health and Family Planning Commission of the PRC
Table of Contents
Foreword . 3 
1 Scope .. 4 
2 Principles . 4 
3 Reagents and materials .. 4 
4 Instruments and equipment . 6 
5 Analytical procedures . 6 
6 Analysis results expression . 7 
7 Precision. 8 
8 Others . 8 
Appendix A High performance liquid chromatograms of maleic acid . 9 
Foreword
This Standard replaces GB/T 23296.21-2009 “Food contact materials - Polymer
- Determination of maleic acid and maleic anhydride in food simulants - High
performance liquid chromatography”.
As compared with GB/T 23296.21-2009, the main changes of this Standard are
as follows.
- The Standard’s name is “National Food Safety Standard - Food Contact
Materials and Articles - Determination of Migration of Maleic Acid and Its
Acid Anhydride”;
- MODIFY the conditions for high performance liquid chromatography;
- MODIFY the limit of detection.
National Food Safety Standard -
Food Contact Materials and Articles - Determination of
Migration of Maleic Acid and Its Acid Anhydride
1 Scope
This Standard specifies the method for determination of migration of maleic acid
and its acid anhydride in food contact materials and articles.
This Standard applies to the determination of the total amount of maleic acid in
food simulants.
2 Principles
Maleic anhydride, in the presence of water, turns into maleic acid. By
determining the content of maleic acid, the migration of maleic acid and its acid
anhydride is indirectly obtained. After the sample is soaked, water-based food
simulants are injected directly. Oil-based food simulants, after being extracted
by sodium bicarbonate solution and purified by C18 solid-phase extraction
cartridge, are determined by high performance liquid chromatography. ADOPT
reversed-phase chromatographic column to separate; ultraviolet detector to
detect; external standard method to quantify.
3 Reagents and materials
Unless otherwise stated, the reagents used in this method are analytically pure;
the water is the Grade I water specified in GB/T 6682. During the test,
containers and transfer instruments shall avoid the use of plastic material.
3.1 Reagents
3.1.1 Methanol (CH4O). chromatographically pure.
3.1.2 Phosphoric acid (H3PO4).
3.1.3 Sodium bicarbonate (NaHCO3).
3.1.4 Reagents required for the preparation of water-based, acidic, alcohol-
food simulants is 0.00 mg/kg, 2.00 mg/kg, 4.00 mg/kg, 8.00 mg/kg, 20.0 mg/kg,
and 40.0 mg/kg, respectively. ADD 25 mL of 1% sodium bicarbonate solution;
SHAKE for 5 min; LET stand for layering; PIPETTE the lower-layer aqueous
solution; USE C18 solid-phase extraction cartridge to filter; TAKE 1.0 mL of the
filtrate, through a 0.45 μm filter membrane, FILTER it in injection bottle; to be
determined by the machine.
4 Instruments and equipment
4.1 High performance liquid chromatography. equipped with diode array
detector or ultraviolet detector.
4.2 Electrothermal constant-temperature dry box.
4.3 Analytical balance. The sensitivity is 1.0 mg and 0.1 mg.
4.4 Vortex mixer.
4.5 C18 solid-phase extraction cartridge. The cartridge stuffing is 500 mg.
5 Analytical procedures
5.1 Preparation of test solution of food simulants
5.1.1 Migration test
According to the requirements of GB 5009.156 and GB 31604.1, CARRY out
migration test on the sample; and OBTAIN test solution of food simulants.
5.1.2 Treatment of soaking solution
5.1.2.1 Preparation of test solution of water-based food simulants
(including distilled water, 4% of acetic acid, alcohol simulants)
TAKE about 1.5 mL of the water-based food simulants obtained from the
migration test; through a 0.45 μm filter membrane, FILTER it for determination.
2 samples are prepared in parallel.
5.1.2.2 Oil-based food simulants
Accurately WEIGH 25 g (accurate to 0.1 g) of soaking solution of oil-based food
simulants in separating funnel; ADD 25 mL of 1% sodium bicarbonate solution;
SHAKE for 5 min; LET stand for layering; PIPETTE the lower-layer aqueous
solution; USE C18 solid-phase extraction cartridge to filter; TAKE 1.0 mL of the
filtrate, through a 0.45 μm filter membrane, FILTER it in injection bottle; to be