GB/T 31270.18-2014 English PDF (GBT31270.18-2014)

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GB/T 31270.18-2014: Test guidelines on environmental safety assessment for chemical pesticides -- Part 18: Amphibian acute toxicity test
GB/T 31270.18-2014
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.100
B 13
Test guidelines on environmental safety assessment
for chemical pesticides -
Part 18. Amphibian acute toxicity test
ISSUED ON. OCTOBER 10, 2014
IMPLEMENTED ON. MARCH 11, 2015
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine;
Standardization Administration Committee.
Table of Contents
Foreword ... 3 
1 Scope ... 5 
2 Terms and definitions ... 5 
3 Test overview ... 6 
4 Test methods ... 6 
5 Test report ... 10 
Appendix A ... 11 
Appendix B ... 12 
References ... 13 
Foreword
GB/T 31270 “Test guidelines on environmental safety assessment for chemical
pesticides” is divided into 21 parts.
- Part 1. Transformation in soils;
- Part 2. Hydrolysis;
- Part 3. Phototransformation;
- Part 4. Adsorption/Desorption in soils;
- Part 5. Leaching in soil;
- Part 6. Volatility;
- Part 7. Bioconcentration test;
- Part 8. Degradation in water-sediment systems;
- Part 9. Avian acute toxicity test;
- Part 10. Honeybee acute toxicity test;
- Part 11. Silkworm acute toxicity test;
- Part 12. Fish acute toxicity test;
- Part 13. Daphnia sp. acute immobilisation test;
- Part 14. Alga growth inhibition test;
- Part 15. Earthworm acute toxicity test;
- Part 16. Soil microorganism toxicity test;
- Part 17. Trichogramma acute toxicity test;
- Part 18. Amphibian acute toxicity test;
- Part 19. Effects on non-target plants;
- Part 20. Livestock short-term dietary toxicity test;
- Part 21. Macro-crustacean toxicity test.
This Part is Part 18 of GB/T 31270.
Test guidelines on environmental safety assessment
for chemical pesticides -
Part 18. Amphibian acute toxicity test
1 Scope
This Part of GB/T 31270 specifies the amphibian acute toxicity test’s material,
conditions, operation, quality control, data processing, test report and other
basic requirements.
This Part is applicable to amphibian acute toxicity test for chemical pesticide
registration. Other types of pesticides may refer for use.
This Part is not applicable to volatile and insoluble chemical pesticides.
2 Terms and definitions
The following terms and definitions are applicable to this document.
2.1 Median lethal concentration
In the acute toxicity test, the concentration of test substance when it causes 50%
of test biology dead, represented in LC50.
Note. The unit is mg a.i./L.
2.2 Test substance
The substance to be tested in the test.
2.3 Chemical pesticide
The pesticide that is synthetically made of chemical substances. Some take
active substance in natural product as parent body to imitate and structurally
transform, which are called biomimetic synthesis pesticides.
Synonym. synthetic organic pesticide
[NY/T 1667.1-2008, Definition 2.3.1]
2.4 Technical material
The final product that is made of active ingredients and impurities obtained in
the manufacturing process. It must not contain visible foreign matters or any
additives. If necessary, add a small amount of stabilizer.
[NY/T 1667.2-2008, Definition 2.5.1]
2.5 Formulation product
The product that is made of pesticide (or parent drug) and additive, and has
stable state of use.
[NY/T 1667.2-2008, Definition 2.4]
2.6 Semi-static test
During the test, replace the liquid at a regular interval (e.g. 24h), so as to
maintain the concentration of the test liquid not less than 80% of the initial
concentration.
2.7 Static test
Do not replace the test liquid during the test.
2.8 Flow-through test
Continuously refresh the test liquid during the test.
3 Test overview
The test methods for amphibian acute toxicity are static test, semi-static test,
and flow-through test. It shall adopt suitable method according to the nature of
the test substance. Respectively prepare different concentrations of the test
substances. Observe and record tadpole’s symptoms of poisoning and number
of deaths every day during 96 h test. And calculate LC50 values and 95%
confidence limits of 24 h, 48 h, 72 h and 96 h respectively.
4 Test methods
4.1 Material and conditions
4.1.1 Test biology
Rana Limnocharis or Xenopus laevis tadpole is recommended. Specific age
and appropriate water temperature can refer to Appendix A. If use other
amphibians as test material, they shall meet corresponding domestication and
test conditions of physiological requirements, and explanation shall be given as
- Mortality rate during the test shall not exceed 10%, and no abnormal
behavior occurs;
- During the test, the dissolved oxygen content of test liquid shall not be less
than 60% of air saturation value (ASV).
5 Test report
Test report shall at least contain the following items.
- Information of test substance, including test pesticide’s common name,
chemical name, structural formula, CAS number, purity, basic physical and
chemical properties, origin, etc.;
- Test biology’s name, origin, size and domesticated situation;
- Test conditions, including test temperature, illumination, dilution water’s
temperature, dissolved oxygen concentration and pH, etc.;
- Test liquid concentration, LC50 value of 24 h, 48 h, 72 h, and 96 h, 95%
confidence limits, and calculation method;
- If tadpoles of the control group die or have abnormal reactions;
- Observed toxic effects, e.g., any abnormal behavior or poisoning symptom
of test tadpoles;
- Classification of amphibian toxicity refers to Appendix B.
Appendix B
(Informative)
Acute toxicity classification of pesticide to tadpole
According to median lethal concentration LC50 (96h) of pesticide to tadpole,
classify the acute toxicity of pesticide to tadpole into four grades, as shown in
Table B.1.
Table B.1 Toxicity classification of pesticides to amphibian
Toxicity classification LC50 (96h) / (mg a.i./L)
Severe toxicity LC50 ≤ 0.1
High toxicity 0.1 < LC50 ≤ 1.0
Medium toxicity 1.0< LC50 ≤ 10
Low toxicity LC50 >10
References
[1] NY/T 1667.1-2008 Terminology of pesticide registration management -
Part 1. Basic terminology
[2] NY/T 1667.2-2008 Terminology of pesticide registration management -
Part 2. Product chemistry
[3] OECD (2009). Guideline 231. The Amphibian Metamorphosis Assay,
OECD Guidelines for the Testing of Chemicals.
[4] US EPA (1996). Tadpole / Sediment Subchronic Toxicity Test (OPPTS
850.1800). Ecological effects test guidelines.
[5] ASTM (2007). Standard Guide for Conducting Acute Toxicity Tests on Test
Materials with Fishes, Macr. Oinvertebrates, and Amphibians. American
Society for Testing and Materials, ASTM E729-96.
[6] OECD (l992). Guideline 203. Fish, Acute Toxicity Test. OECD Guidelines
for the Testing of Chemicals.
[7] Cai Daoji. Research on Pesticide Environmental Toxicology. Beijing. China
Environmental Science Press, 1999.