GB 1886.170-2016 English PDF (GB1886.170-2016)

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GB 1886.170-2016: Food safety national standard -- Food additive - 5-guanylate disodium
GB 1886.170-2016
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard -
Food Additives - 5'-guanylate disodium
食品添加剂 5'-鸟苷酸二钠
ISSUED ON: AUGUST 31, 2016
IMPLEMENTED ON: JANUARY 01, 2017
Issued by: National Health and Family Planning Commission of the PRC
Table of Contents
1 Scope ... 3 
2 Chemical name, structural formula, molecular formula, and relative molecular
mass ... 3 
3 Technical requirements ... 3 
Appendix A Inspection methods ... 5 
National Food Safety Standard -
Food Additives - 5'-guanylate disodium
1 Scope
This Standard applies to the food additive 5'-guanylate disodium prepared by
fermentation or enzymatic hydrolysis using starch and saccharide as raw
materials.
2 Chemical name, structural formula, molecular
formula, and relative molecular mass
2.1 Chemical name
5'-guanylate disodium
2.2 Structural formula
2.3 Molecular formula
C10H12N5Na2O8P • nH2O
2.4 Relative molecular mass
407.18 (according to 2011 international relative atomic mass, on anhydrous
basis)
3 Technical requirements
3.1 Sensory requirements
Spectrophotometer.
A.3.2 Analytical procedures
WEIGH 1 g of sample (accurate to 0.0001 g); dissolve in 20 mL of water; shake
well. PLACE the above sample solution into a 1 cm cuvette; USE water as a
blank control; USE a spectrophotometer to, at 430 nm, determine the light
transmittance of the sample solution; RECORD the reading.
The test result is based on the arithmetic mean of parallel determination results.
The absolute difference between the two independent determination results,
obtained under repeated conditions, shall not exceed 0.5% of the arithmetic
mean.
A.4 Determination of pH (5% aqueous solution)
A.4.1 Instruments and equipment
Acidimeter.
A.4.2 Analytical procedures
WEIGH 1 g of sample (accurate to 0.0001 g); dissolve in 20 mL of water; USE
an acidimeter to determine the pH of the above sample solution.
The test result is based on the arithmetic mean of parallel determination results.
The absolute difference between the two independent determination results,
obtained under repeated conditions, shall not exceed 0.2.
A.5 Determination of other nucleotides
A.5.1 Reagents and materials
A.5.1.1 Developing solution: tert-butanol+saturated ammonium sulfate+0.025
mol/L aqueous ammonia=3+160+40.
A.5.1.2 Chromatographic filter paper.
A.5.2 Instruments and equipment
A.5.2.1 UV analyzer.
A.5.2.2 Microinjector.
A.5.3 Analytical procedures
WEIGH 1.0 g of sample; ADD water to dissolve and dilute to 100 mL. USE a
chromatographic filter paper for application of 10.0 μL of sample; SPREAD