GB 1886.318-2021 English PDF (GB1886.318-2021)

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GB 1886.318-2021: National food safety standard - Food additives - Zeaxanthin
GB 1886.318-2021
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard - Food Additives -
Zeaxanthin
ISSUED ON: FEBRUARY 22, 2021
IMPLEMENTED ON: AUGUST 22, 2021
Issued by: National Health Commission of the People’s Republic of China;
State Administration for Market Regulation.
Table of Contents
1 Scope ... 3
2 Chemical Name of Main Components, Molecular Formula, Structural Formula
and Relative Molecular Mass ... 3
3 Technical Requirements ... 3
Appendix A Inspection Methods ... 5
Appendix B Liquid Reference Chromatograms of Zeaxanthin, Cryptoxanthin
and Lutein Standard Solutions ... 9
National Food Safety Standard - Food Additives -
Zeaxanthin
1 Scope
This Standard is applicable to corn and/or food additive of zeaxanthin that takes the
corn as raw materials, and is prepared through extracting and concentrating the
extraction solvents of vegetable oil.
2 Chemical Name of Main Components, Molecular
Formula, Structural Formula and Relative Molecular
Mass
2.1 Molecular formula
Zeaxanthin: C40H56O2
2.2 Structural formula
Zeaxanthin:
2.3 Relative molecular mass
Zeaxanthin: 568.87 (as per 2016 international relative atomic mass)
3 Technical Requirements
3.1 Sensory requirements
The sensory requirements shall comply with the provisions of Table 1.
Appendix A
Inspection Methods
A.1 General provisions
If no other requirements are specified, all the reagents and water used in this Standard
refer to the analytical reagents and Class-III water specified in GB/T 6682. If no other
requirements are specified, all the standard solution used in the test, standard solution
for determining the impurity, preparation and product shall be prepared according to
the provisions of GB/T 601, GB/T 602, GB/T 603.The solution used in the test refers
to the aqueous solution when the solvent is not specified.
A.2 Identification test
Take an appropriate amount of specimen and dissolve it in chloroform. The specimen
solution shall have a maximum absorption peak in the wavelength range of 455nm ±
10nm.
A.3 Determination of colour value
A.3.1 Reagents and materials
Chloroform.
A.3.2 Apparatus
Ultraviolet-visible spectrophotometer.
A.3.3 Analysis procedures
Accurately weigh 0.1g of specimen (accurate to 0.0001g), dissolve it by chloroform;
transfer to a 100mL volumetric flask; add chloroform to make constant volume to the
mark; and shake well. Use this solution as a specimen solution; place it in a 1cm
cuvette; use chloroform as a blank control; and use an ultraviolet-visible
spectrophotometer to measure the absorbance (the absorbance shall be controlled at
0.3~ 0.7; otherwise, the concentration of the specimen solution shall be adjusted; and
then the absorbance shall be re-measured) at the maximum absorption wavelength of
455nm±10nm.
A.3.4 Calculation of results
The colour value E1% 1cm (455nm±10nm) is calculated according to Formula (A.1).